Figure 4
Expression of β-galactosidase driven by HIV-1-LTR in HIV-infected cells after siRNA transfection. P4-CCR5 cells containing a HIV-1-LTR driven β-galactosidase reporter vector were transfected with siGliPR-2, non-silencing control siRNA or no siRNA (mock) and subsequently infected with HIV-1LAI with a multiplicity of infection of 0.01. (A) β-galactosidase units at day 4 normalized by relative WST-1 values represent mean values from triplicate samples. (B) Photomicrograph of β-gal stained P4-CCR5 cells infected with HIV-1LAI (MOI 0.01) after transfection with mock, GliPR-siRNA, HIV-1 p24-siRNA or non-silencing control siRNA at day 6 post infection.