Figure 3
Effects of siRNA transfections on HIV-1 replication. P4-CCR5 cells were transfected with siRNAs directed against GliPR, viral p24 or an unspecific sequence (non-silencing control) and subsequently infected with HIV-1LAI with a multiplicity of infection of 0.01 (A) and 0.05 (B), respectively. HIV-1 RNA copy numbers were normalized per cell count by house keeping gene GAPDH. (C) P4-CCR5 cells were transfected with siGliPR-2, si-p24 or non-silencing control siRNA and subsequently infected with HIV-1LAI with a multiplicity of infection of 0.01. Concentrations of viral p24 at day 0, 2 and 4 represent mean values of triplicate samples. (D) Jurkat cells were transfected with siRNAs directed against GliPR, viral p24 or an unspecific sequence (non-silencing control) and subsequently infected with HIV-1LAI with a multiplicity of infection 0.01. HIV-1 RNA copy numbers were normalized per cell count by house keeping gene GAPDH.