Figure 2

Efficacy of siRNA-mediated suppression of GliPR. (A) Quantitative PCR analysis of GliPR expression in P4-CCR5 cells which were transfected with 2 different siRNAs against GliPR or the control non-silencing siRNA labeled with rhodamine. Results are presented as mean values of triplicate samples ± standard deviation (SD). (B) Cell viability was determined with the WST-1 assay 24 h and 48 h after siRNA transfection. Results are expressed as absorbance (OD₄₅₀). Bars represent the standard deviation of the mean of determinations. (C) Quantitative PCR analysis of GliPR expression in Jurkat cells 2 days after transfection with 2 different siRNAs against GliPR or the control non-silencing siRNA labeled with rhodamine.