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Figure 3 | Retrovirology

Figure 3

From: Cysteine 95 and other residues influence the regulatory effects of Histidine 69 mutations on Human Immunodeficiency Virus Type 1 protease autoprocessing

Figure 3

Different substitutions of H69 have differential effects on protease maturation. (A) HEK 293T cells grown on 6-well plates were transfected with the indicated proviral DNAs by calcium phosphate. The total cell lysates and VLPs were prepared as described (Material and Methods) and subjected to western blot analysis. Mouse monoclonal anti-p24 antibody was used to detect p24-containing proteins (p55, p25, and p24) in the transfected cells and the released VLPs. The cell lysates blot was stripped and reprobed for GAPDH as loading controls. VLP associated proteases were probed with polyclonal rabbit anti-PR antibodies. (B) Relative Gag processing efficiencies were quantified from three independent experiments and the bars represent standard deviations.

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