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Figure 3 | Retrovirology

Figure 3

From: GPG-NH2acts via the metabolite αHGA to target HIV-1 Env to the ER-associated protein degradation pathway

Figure 3

αHGA acts on gp160 independently of supplemented serum in cell culture medium. HeLa-tat III cells were cultured in cell culture medium supplemented with 10% FBS and transfected to express gp160 for 20 h. Two hours upon transfection the cell culture supernatants were carefully removed, the cells rinsed twice in PBS and provided with culture medium containing either 10% HS (upper panel) or no serum (lower panel) and indicated concentrations of GPG-NH2, G-NH2 or αHGA. The cell lysates were immunoblotted with mAb towards gp41.

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