Figure 3
From: GCN5-dependent acetylation of HIV-1 integrase enhances viral integration
IN interacts with GCN5 both in vitro and in vivo. (A) Extracts from HEK 293T cells transfected with the indicated plasmids were immunoprecipitated using anti-Flag antibody and analyzed by Western blotting with anti-HA antibody (upper panel) or anti-Flag antibody (middle panel). Lower panel: extracts immunoblotted with anti-HA antibody. (B) Extracts from HEK 293T cells transfected with the indicated plasmids were immunoprecipitated using anti-HA antibody and analyzed by Western blotting with anti-Flag antibody (upper panel) or anti-HA antibody (middle panel). Lower panel: extracts immunoblotted with anti-Flag antibody. (C) Autoradiography and Coomassie Blue staining of in vitro binding assays with GST-GCN5 and 35S-IN or the indicated 35S-IN fragments. The histogram represents the results of three independent experiments (means ± SEM), where the amounts of bound proteins are expressed as percentages of the corresponding radiolabeled inputs. Statistical significance of the binding percentages was calculated by using the Student's two-sided t test. Asterisks directly above bars indicate differences in binding efficiency to GST-GCN5 between IN deleted forms and full-length IN. **, P < 0,01; *, P < 0,05. Conversely, where asterisks are not present, values obtained did not significantly differ (P > 0,05) from those obtained with control, non-silenced cells.