Figure 6
From: Anti-tetherin activities in Vpu-expressing primate lentiviruses
Anti-tetherin activity of SIVsyk lineage Vpu proteins. (A) SIVsyk lineage of the primate lentiviruses, showing viruses that express Vpu (boxed). SIVmon/mus/gsn viruses form the SIVgsn sublineage, while SIVden is less closely related. Figure adapted from Dazza et al. (2005) [28]. (B) Expression of SIVmus/mon/gsn and SIVden Vpu-EGFP proteins detected with anti-GFP antibody. Anti-tetherin activities of indicated Vpu-EGFP proteins were measured by HIV-1 VLP release assays, against (C) human tetherin present in HeLa cells, (D) Mac-tetherin expressed in macaque (LLCMK2) cells, and (E) Hum-tetherin expressed in LLCMK2 cells. HIV-1 Vpu and SIVmac239 Nef-EGFP proteins were included as controls. Mean fold-enhancement of HIV-1 VLP release in presence of anti-tetherin proteins is shown relative to baseline (control) levels, for n = 2 independent experiments.