Figure 2
From: Anti-tetherin activities in Vpu-expressing primate lentiviruses
Activity of HIV-1 and SIVcpz Vpu against chimpanzee tetherin. Anti-tetherin activities of indicated viral proteins were examined by measurement of HIV-1 VLP release, detected by Western blotting of cell lysate and VLP fractions with anti-p24 antibody (left panels) or as mean-fold enhancement of VLP release relative to baseline (control) levels in absence of Vpu or Env proteins (right panels): (A) HIV-1 Vpu and HIV-2 Env activity against human (Hum) and chimpanzee (Cpz) tetherin expressed in 293A cells, (B) Activity of HIV-1 Vpu and SIVcpz GAB1 and SIVcpz ANT Vpu-EGFP proteins against Cpz-tetherin expressed in 293A cells, and (C) Activity of HIV-1 Vpu and SIVcpz GAB1 and SIVcpz ANT Vpu-EGFP proteins against Cpz-tetherin expressed in chimpanzee (Cpz_B) cells. * indicates p24 signal was too low to quantify.