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Figure 1 | Retrovirology

Figure 1

From: Anti-tetherin activities in Vpu-expressing primate lentiviruses

Figure 1

HIV-1 but not SIVcpz Vpu overcomes human tetherin restriction. (A) SIVcpz/HIV-1 lineage of the primate lentiviruses, showing three major HIV-1 groups (M, N and O) and the SIVcpz isolates used in this study. SIVcpz TAN3 and ANT were isolated from Pan troglodytes schweinfurthii (P.t.s.) and are less closely related to HIV-1 than SIVcpz strains isolated from Pan troglodytes troglodytes (P.t.t.). Figure adapted from Wain et al. (2007) [35]. (B) Confocal analysis of distribution of GAB1 and ANT Vpu-EGFP fusion proteins and EGFP control, in transiently transfected HeLa cells. (C) HeLa cells (express tetherin) were transfected with pHIV-1-pack (expresses HIV-1 Gag-Pol, Rev), together with either a control CMV expression vector (-), or expression plasmids for human codon-optimized Vpu from HIV-1 (HIV-1 Vpu), or non-codon-optimized EGFP tagged Vpu proteins from HIV-1, SIVcpz GAB1 or SIVcpz ANT. Cell lysates (lys) were probed with indicated antibodies. The Vpu-EGFP proteins from HIV-1, SIVcpz GAB1 and SIVcpz ANT have predicted molecular weights of 47, 33 and 42 kDa, respectively. Intracellular Gag proteins in cell lysates and virus-like particles released into supernatant (VLP) were detected using anti-p24 antibody. Mean-fold enhancement of HIV-1 VLP release in presence of Vpu is shown relative to baseline (control) levels in absence of Vpu for three independent experiments, except for the HIV-1 Vpu-EGFP sample (n = 1).

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