In situ detection of Gag-specific CD8+cells in the GI tract of SIV infected Rhesus macaques

Retrovirology

Table 2 SIV DNA and RNA quantification.

ID No	Specimen	SIV RNA copy eq/mL	SIV DNA copies/10,000 cells	SIV RNA copies/250 ng total RNA
RM 1	Plasma	50
RM 2	Plasma	3.3 × 10⁶
RM 3	Plasma	< 30
RM 1	Spleen		0	0
RM 2	Spleen		142	15986
RM 3	Spleen		0	0
RM 1	Submandibular LN		9	120
RM 2	Submandibular LN		391	29155
RM 3	Submandibular LN		19	28
RM 1	Mesenteric LN		10	7
RM 2	Mesenteric LN		283	9736
RM 3	Mesenteric LN		18	324
RM 1	Ileum		ND	ND
RM 2	Ileum		14	74
RM 3	Ileum		0	0
RM 1	Colon		0	0
RM 2	Colon		48	18755
RM 3	Colon		0	0

Plasma SIV RNA was assessed using a real-time RT-PCR assay and SIV RNA and DNA from lymphoid tissue biopsies were assayed by RT-PCR and PCR. Biopsy RNA and DNA were amplified with the Rhesus Monkey GAPDH kit (Applied Biosystems Inc) to confirm the nucleic acids were amplifiable (data not shown). LN; lymph node and ND; not done due to lack of material.

ISSN: 1742-4690