Skip to main content

Table 1 Percentage of Gag CM9 positive cells quantified by flow cytometry analysis of single cell suspension.

From: In situ detection of Gag-specific CD8+cells in the GI tract of SIV infected Rhesus macaques

ID No

Specimen

Total counts

Live lymphocyte cell counts

CD3+CD8+cell counts

% CD3+CD8+of lymphocytes

Gag CM9+cell counts

% Gag CM9 of CD3+CD8+cells

RM 1

Spleen

100 000

57 909

14 254

24.60%

1 273

8.93%

RM 2

Spleen

100 000

63 112

13 287

21.10%

1 101

8.29%

RM 3

Spleen

100 000

71 298

21 147

29.70%

2 416

11.40%

RM 1

Mesenteric LN

50 000

42 766

10 116

24.00%

509

5.03%

RM 2

Mesenteric LN

100 000

65 733

15 179

23.10%

580

3.82%

RM 3

Mesenteric LN

100 000

40 436

13 546

33.50%

701

5.17%

RM 1

PBMC

65 793

29 501

6 712

22.80%

438

6.52%

RM 2

PBMC

100 000

45 200

14 287

31.60%

249

1.74%

RM 3

PBMC

100 000

69 346

21 362

30.80%

776

3.63%

  1. The total cell count, live lymphocyte cell count, CD3+CD8+ cell counts, percentage of CD3+CD8+ cells in the live lymphocyte population, Gag CM9+ cell counts and percentage of Gag CM9+ cell in the CD3+CD8+ population was calculated by essaying flow staining on single cell suspension of spleen, mesenteric lymph nodes and PBMCs. Cells were gated on live CD3+CD8+ cells. LN; lymph node