Figure 5
From: In situ detection of Gag-specific CD8+cells in the GI tract of SIV infected Rhesus macaques
Gag CM9 T cells are located in the extra follicular area of lymphoid tissue. Fluorescence images showing sections from an SIV infected Mamu-A*01 positive RM. A-C) Images of a solitary lymphoid follicle in a colon section. A) Gag CM9 positive cells are located in the extra follicular region. Gag CM9 Qdot 655 multimer (red), CD20 (green). B) No CD8+ cells were located in the extra follicular region. CD8 (red), CD20 (green). C) All CM9 positive cells were CD8+. Gag CM9 Qdot 655 multimer (red), CD8 (green). D) Image of a mesenteric lymph node section showing that the majority of the Gag CM9 positive cells are located in the extra follicular region, but some are in the junction of the extra follicular and follicular area. Gag CM9 Qdot 655 multimer (red), CD20 (green) E) A magnified view of the region indicated in panel D showing the Gag CM9 positive cells the junction of the extra follicular and follicular area. All sections were stained with DAPI (blue). Images were collected with a 20×/0.75 objective. Scale bar = 50 μm.