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Figure 4 | Retrovirology

Figure 4

From: In situ detection of Gag-specific CD8+cells in the GI tract of SIV infected Rhesus macaques

Figure 4

In situ staining of CD8+Gag CM9+ cells in frozen tissue. A) Fluorescence images of a spleen tissue section from an SIV infected Mamu-A*01 positive RM stained with CD8 (green), Gag CM9 or FLP Qdot 655 multimer (red), and DAPI (blue) demonstrating that the Gag CM9 positive cells are CD8+. Fluorescence images of B) spleen sections, C) mesenteric lymph node sections, D) ileum sections with peyer's patch, E) solitary lymph node in colon and F) images of lamina propria in colon tissue from SIV infected Mamu-A*01 positive RM. Left-most column, second column, and right most column show Gag CM9 Qdot 655 multimer (red). The second column is a magnification of the left column images. Third column; FLP Qdot 655 multimer (red). Right-most column, similar sections from a non-infected Mamu-A*01 negative RM. Images were collected with a 20×/0.75 objective. Scale bar = 50 μm.

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