Figure 6

Tetherin cell surface expression on target cells promotes viral cell-to-cell transfer and transmission. Tetherin^pos or tetherin^neg Sup-T1 effector cells, infected with wt or Δvpu virus, were co-cultured with tetherin^pos or tetherin^neg Sup-T1 target cells. Target cells were assessed for viral transfer and viral transmission by flow cytometry analysis for CA p24 and GFP expression, respectively. Data are normalized for tetherin^pos target cells in each case. Data are derived from three independent experiments; error bars represent SEM. (A & B). Viral transfer (CA p24) of wt and Δvpu virus from tetherin^pos (A) and tetherin^neg (B) effector cells. (C & D). Viral transmission (GFP) of wt and Δvpu virus from tetherin^pos (C) and tetherin^neg (D) effector cells.