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Figure 4 | Retrovirology

Figure 4

From: Tetherin restricts direct cell-to-cell infection of HIV-1

Figure 4

Co-culture strategy and flow cytometry analysis. Effector cells (grey) were infected with wt or Δvpu virus, cultured for 48 h, washed and then co-cultured with uninfected target cells, which were stained with CMAC (blue) either together or separated by a virus permeable membrane (3 μm pore size). Viral transfer was assessed by flow cytometry analysis of viral CA p24 protein (yellow) in target cells 6 h after the initiation of co-culture. Viral transmission was assessed 24 h later by flow cytometry analysis for GFP expression (green) in target cells.

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