Figure 2

Tetherin co-localizes with actin at cell-cell contact zones. Confocal microscopy of co-cultured infected and uninfected tetherin^pos cells, stained for actin, cell surface tetherin, and GFP, presented as single stains (A-C) and in an overlay image (D). Tetherin expression was induced by 100 ng/ml doxycycline and detected using Alexa 647-labeled secondary antibody directed against a primary anti-tetherin antibody (B). Actin was stained with Alexa 594 phalloidin (A); the virus derived GFP signal was amplified by GFP specific Alexa 488-labeled antibody (C).