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Table 2 Screening human cell line genomic DNA using Taqman PCR

From: Disease-associated XMRV sequences are consistent with laboratory contamination

Cancer cell line

Taqman PCR Result (Ct FAM)

Sequences obtained

Name

COSMIC ID

Cancer type

XMRV -

gag-leader

XMRV -int

MLV-X -gag

gag

pol

env

A2780

906804

Ovary adenocarcinoma

No Ct

No Ct

27

S

S

S

BHY

753535

Squamous cell carcinoma

No Ct

No Ct

15

S

S

S

CoCM-1

910783

Colon adenocarcinoma

16

No Ct

16

S

S

M

Daudi

906831

Lymphoid neoplasm/Burkitt lymphoma

21

No Ct

22

M

M

S

EKVX

905970

Lung adenocarcinoma

No Ct

No Ct

18

M

S

S

IMR-5

907170

Neuroblastoma

No Ct

No Ct

20

S

S

S

MUTZ-1

908155

Haematopoietic neoplasm/Myeloid leukaemia

40

No Ct

14

S

S

S

S-117

910946

Thyroid sarcoma

19

No Ct

18

M

S

S

TYK-nu

909774

Ovary carcinoma

27

No Ct

18

M

S

S

  1. Only MLV-X-gag positive cell lines are included. A total of 411 DNAs from human tumour cell lines from the COSMIC (Catalogue Of Somatic Mutations In Cancer repository) http://www.sanger.ac.uk/genetics/CGP/cosmic representing a variety of cancer types were screened. Common names, (Cosmic ID) and tumour type are shown. Cycle threshold (Ct) values for the three Taqman PCR reactions are shown. (S) denotes a single sequence (M) denotes multiple sequences obtained after direct sequencing of gag, pol and env PCR products. We define positive PCR as those with a Ct of less than 41. Primers are shown in Table S1.