Figure 2
From: Contamination of human DNA samples with mouse DNA can lead to false detection of XMRV-like sequences
Sensitivity of nested PCR for XMRV gag. A) Titration of genomic DNA from WPI-1282. PCR amplicons from 83.3, 13.8, 2.3, 0.3, 0.05 and 0 cell equivalents of genomic DNA from the WPI-1282 cell line in the presence of 3.3 × 104 cell equivalents of LNCaP genomic DNA were run on a 1.5% agarose gel to show the sensitivity of the assay. gapdh was used as the loading control. XMRV gag yields an expected product of 413 bp. NTC = No Template Control. B) Representative example of nested PCR for XMRV gag. Sample TH03.1.1 was first tested at 3.3 × 104 cell equivalents of genomic DNA, followed by limiting dilutions of 1.1 × 104 and 3.7 × 103 cell equivalents. Once a dilution had 1 out of 3 samples positive for gag, the positive band was purified and sequenced.