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Figure 1 | Retrovirology

Figure 1

From: Mouse DNA contamination in human tissue tested for XMRV

Figure 1

Nested PCR on DNA extracted from FFPE tissue of prostate cancer patients. Figure (a) shows samples that produced a PCR product of the expected size using primers specific for XMRV (lanes 1-5). UK patient 308 and UK 244 (Lane 1 and 2); Thailand 1 and Thailand 2 (Lane 3 and Lane 4); Korea 62 (Lane 5). Lanes 6-8 show samples in which XMRV was not detected. Thailand 3 (Lane 6); Korea 60 (Lane 7); Korea 61(Lane 8); Positive control pXMRV produces a strong band (lane 9). Promega 200 bp DNA step ladder (lane 10). Lanes 11-13 show water negative controls. Figure (b) shows β-globin control PCR used to demonstrate the presence of human DNA in each sample (lanes 1-8, as above). Expected size was 104 bp. Positive control LNCaP DNA is shown in lane 9. Lanes 11-13 show negative water controls. All patient samples tested showed a positive signal for β-globin. Figure (c) shows the IAP PCR result for the same samples (lanes 1-5 IAP positive, lanes 6-8 IAP negative). Positive control McCoy cell DNA is shown in lane 9. Invitrogen 100 bp DNA step ladder (lane 10). Lanes 11-13 show negative water controls. Figure (d) shows the mtDNA PCR results for the same sample (lane 1 mtDNA positive, lanes 2-8 mtDNA negative) Positive control McCoy cell DNA is shown in lane 9. Invitrogen 100 bp DNA step ladder (lane 10). Lanes 11-13 show negative water controls.

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