Figure 1

Detection of SFV-specific antibodies by Western blot analysis in mandrill and human plasma samples. Seropositivity was defined by the presence of reactivity to the Gag doublet of 70 kDa and 74 kDa as shown for positive controls (CTRL+). Seronegativity was defined as no bands of the gag doublet observed by Western blot, as in the negative control (CTRL-). Reactivity with a single band in the 70- to 74-kDa molecular mass range was considered indeterminate. The mandrills samples Mnd5DCP, F 19y; Mnd5D3CDP, F 11y; Mnd17A9CDP, M 4y; Mnd2D9CDP, M 6y; Mnd10E5CDP, F 5y; Mnd2DCDP, F 20y; Mnd5MCDP, M 9y; MndNB, M 5y; Mnd17HCDP, M 10y; Mnd12D3CDP, F 15y; Mnd5D3B, F 4y; Mnd2D8CDP, M 7y; Mnd16G2CDP, F 4y; Mnd16iCDP, M 8y and human H1CIRMF and H2CIRMF are seropositive. Only mandrills Mnd2DCDP, MndNB and Mnd5D3B were negative in PCR. The mandrill Mnd17D7CDP, M 4y and the human H3CIRMF are indeterminate; and mandrills Mnd17F4CDP, F 4y and Mnd12O2CDP are seronegative. Mnd: mandrill; CDP: Centre de Primatologie; in the middle: mandrill identity; M: male; F: female; Y: years. The relative molecular masses of SFVcpz-specific Gag protein are indicated on the left (MW). The Western blot positive control is a serum from an SFV-positive chimpanzee [16]. The negative serum was obtained from a person who had never been in contact with a nonhuman primate. H1CIRMF, H2CIRMF, and H3CIRMF are the results of Western blot serology for human samples.