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Table 1 Plasmids used in this study

From: Membrane topology analysis of HIV-1 envelope glycoprotein gp41

Plasmids Description
For prokaryotic system  
mpKMalp2e-GFP Multiple cloning site-modified pMalp2e containing KanR and Green fluorescent protein genes
mpKMalp2e-gp41-1-GFP mpKMalp2e-GFP with C-terminally truncated gp41 at W666
mpKMalp2e-gp41-2-GFP mpKMalp2e-GFP with C-terminally truncated gp41 at I682
mpKMalp2e-gp41-3-GFP mpKMalp2e-GFP with C-terminally truncated gp41 at G694
mpKMalp2e-gp41-4-GFP mpKMalp2e-GFP with C-terminally truncated gp41 at R725
mpKMalp2e-gp41-5-GFP mpKMalp2e-GFP with C-terminally truncated gp41 at R747
mpKMalp2e-gp41-6-GFP mpKMalp2e-GFP with C-terminally truncated gp41 at R788
mpKMalp2e-gp41-7-GFP mpKMalp2e-GFP with C-terminally truncated gp41 at A819
mpKMalp2e-gp41-8-GFP mpKMalp2e-GFP with full-length gp41
optGFP1-11/pET-47md Modified pET-47b with modified super folder GFP
For mammalian system  
pHIVenv-Halo The CMV promoter driven mammalian expression vector containing HaloTag gene
pHIVenv-gp41-4-Halo pHIVenv-Halo containing Env with C-terminally truncated gp41 at R725
pHIVenv-gp41-5-Halo pHIVenv-Halo containing Env with C-terminally truncated gp41 at R747
pHIVenv-gp41-6-Halo pHIVenv-Halo containing Env with C-terminally truncated gp41 at R788
pHIVenv-gp41-7-Halo pHIVenv-Halo containing Env with C-terminally truncated gp41 at A819
pHIVenv-gp41-8-Halo pHIVenv-Halo containing full-length Env
pHIVenv-gp41-5 Halo-deleted pHIVenv-gp41-5-Halo
pHIVenv-gp41-8 Halo-deleted pHIVenv-gp41-8-Halo
pHIVenv-CD22-gp41-5 The gp41 MSD replaced pHIVenv-gp41-5 with the MSD of CD22
pHIVenv-CD22-gp41-8 The gp41 MSD replaced pHIVenv-gp41-8 with the MSD of CD22
pHook-Halo-GPI The expression vector of the GPI anchored-HaloTag
pKcTac-Halo The expression vector of Tac antigen of IL-2 receptor fused with C-terminal HaloTag
pKcTac-FLAG pKcTacHalo vector whose HaloTag was replaced with 3xFLAG