Table 1 Plasmids used in this study
From: Membrane topology analysis of HIV-1 envelope glycoprotein gp41
Plasmids | Description |
|---|---|
For prokaryotic system | Â |
mpKMalp2e-GFP | Multiple cloning site-modified pMalp2e containing KanR and Green fluorescent protein genes |
mpKMalp2e-gp41-1-GFP | mpKMalp2e-GFP with C-terminally truncated gp41 at W666 |
mpKMalp2e-gp41-2-GFP | mpKMalp2e-GFP with C-terminally truncated gp41 at I682 |
mpKMalp2e-gp41-3-GFP | mpKMalp2e-GFP with C-terminally truncated gp41 at G694 |
mpKMalp2e-gp41-4-GFP | mpKMalp2e-GFP with C-terminally truncated gp41 at R725 |
mpKMalp2e-gp41-5-GFP | mpKMalp2e-GFP with C-terminally truncated gp41 at R747 |
mpKMalp2e-gp41-6-GFP | mpKMalp2e-GFP with C-terminally truncated gp41 at R788 |
mpKMalp2e-gp41-7-GFP | mpKMalp2e-GFP with C-terminally truncated gp41 at A819 |
mpKMalp2e-gp41-8-GFP | mpKMalp2e-GFP with full-length gp41 |
optGFP1-11/pET-47md | Modified pET-47b with modified super folder GFP |
For mammalian system | Â |
pHIVenv-Halo | The CMV promoter driven mammalian expression vector containing HaloTag gene |
pHIVenv-gp41-4-Halo | pHIVenv-Halo containing Env with C-terminally truncated gp41 at R725 |
pHIVenv-gp41-5-Halo | pHIVenv-Halo containing Env with C-terminally truncated gp41 at R747 |
pHIVenv-gp41-6-Halo | pHIVenv-Halo containing Env with C-terminally truncated gp41 at R788 |
pHIVenv-gp41-7-Halo | pHIVenv-Halo containing Env with C-terminally truncated gp41 at A819 |
pHIVenv-gp41-8-Halo | pHIVenv-Halo containing full-length Env |
pHIVenv-gp41-5 | Halo-deleted pHIVenv-gp41-5-Halo |
pHIVenv-gp41-8 | Halo-deleted pHIVenv-gp41-8-Halo |
pHIVenv-CD22-gp41-5 | The gp41 MSD replaced pHIVenv-gp41-5 with the MSD of CD22 |
pHIVenv-CD22-gp41-8 | The gp41 MSD replaced pHIVenv-gp41-8 with the MSD of CD22 |
pHook-Halo-GPI | The expression vector of the GPI anchored-HaloTag |
pKcTac-Halo | The expression vector of Tac antigen of IL-2 receptor fused with C-terminal HaloTag |
pKcTac-FLAG | pKcTacHalo vector whose HaloTag was replaced with 3xFLAG |