Figure 6

Modulation of tetherin and CD4 in primary T-cells and macrophages by Vpu. (A) FACS analysis of CD4 and tetherin modulation in infected PBL cultures. PBL were infected with HIV-1 variants expressing eGFP via an IRES. Cells were stained with antibodies and measured by flow cytometry three days later. To quantify modulation of cell surface expressed CD4 and tetherin MFI of PBLs infected with HIV-1 NL4-3 WT was set as 100%. Depicted are means +/- SD derived from experiments with four different donors. (B) Primary macrophages were infected with the indicated R5-tropic virus stocks expressing eGFP via an IRES. Cells were analyzed for cell surface MHC-I, CD4 and tetherin five days post infection similar to the PBL cultures. Presented are means +/- SD from infections with macrophages from three different donors each of those were infected with two independent virus stocks.