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Figure 1 | Retrovirology

Figure 1

From: Vpu serine 52 dependent counteraction of tetherin is required for HIV-1 replication in macrophages, but not in ex vivo human lymphoid tissue

Figure 1

Mutation of S52A impairs Vpu-mediated degradation of CD4 and tetherin. (A) Western blot analysis of Vpu expression in lysates of transfected 293T cells. (B) FACS analysis of CD4 expression by Jurkat (upper panel) and CD4 co-transfected 293T cells (lower panel) expressing GFP alone or together with the Vpu and Vpu S52A proteins. Numbers give the MFI of the specified region (C) Quantitative analysis of CD4 downmodulation in Jurkat and 293T cells. Shown are the mean percentages of CD4 down-modulation +/- SD from six (Jurkat) and three (293T) independent experiments. Cell surface CD4 is given as a percentage of that measured on cells transfected with the control vector expressing GFP only (100%). (D) Quantitative analysis of tetherin degradation in 293T cells. Numbers give percentages of GFP+/eCFP+ cells in the specified region. Shown are the mean percentages of tetherin degradation from eight independent transfections. Values give percentages of cells co-expressing GFP and eCFP-tetherin. The mean values obtained with the GFP only control are set as 100%. (E) The same experimental setup as presented in D, however with different concentrations of the indicated CK-II inhibitors added during media change following transfection. Means and standard deviations are calculated from three to six independent transfection experiments.

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Retrovirology

ISSN: 1742-4690

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