Floatation analysis of A3G. HeLa cells were transfected with vectors encoding untagged and C-terminally Myc- or HA-tagged A3G wt (panels 1 - 3), A3G W127A (panels 4 - 6), or A3G Y124 (panels 7-8). The packaging incompetent A3G C100S-Myc variant was included for comparison (panel 9). Cellular caveolin was used as a raft marker (panel 10) and transferrin receptor (TfR) was included as a non-raft associated control (panel 11). Samples were processed for floatation analysis as described in Methods and 10 equal fractions were collected from the top of the gradient. The position of raft and non-raft proteins is indicated at the bottom. Proteins are identified on the right.