Figure 5
From: Biochemical and virological analysis of the 18-residue C-terminal tail of HIV-1 integrase
LEDGFp75-dependent concerted integration activities of WT and IN mutant proteins. (A) The scanned ethidium-stained agarose gels reveal the migration positions of donor, supercoiled (s.c.), and open circular (o.c.) substrate DNAs, as well as half-site and concerted integration reaction products. Donor DNA was omitted from the reactions analyzed in lanes 1 and 25, whereas IN was omitted from lanes 2 and 26. The remaining lanes contained the indicated IN proteins and, at times, LEDGFp75. The concentration of HIV-1/2 IN in lanes 29 and 30 was 1.6 μM, whereas all other IN concentrations were 0.8 μM. The migration positions of molecular mass standards in kb are shown to the left of the gel. (B and C) Levels of overall and concerted DNA strand transfer activities, respectively, normalized to INWT (set to 100%). Results are mean ± SEM for two independent experiments.