Figure 3
Drug effects on luciferase expression from an HIV-1 molecular clone. A. Comparison of drug effects on luciferase expression from the pNL4-3-LucE- molecular clone in 293T cells. The molecular clone pNL4-3-LucE- and pCMV-Ren were transfected into 293T cells. Drugs were added where indicated at the following concentrations: P2 (30 μM), CPX (30 μM), DEF (250 μM), or DFOX (15 μM). Dual luciferase assays were conducted at 12 hr post-transfection. Firefly (FF) luciferase expression was normalized to Renilla luciferase (Ren) from pCMV-Ren (mean of 2 experiments in duplicate, ± SD). B. Expression in Jurkat cells was assayed essentially as in panel A. C. Firefly and Renilla luciferase RNA expression was analyzed in 293T cells treated as in panel A by RPA using 32P- [UTP] labeled antisense RNA probes corresponding to the C-termini of the FF and Ren luciferase mRNAs. D. Comparison of drug effects on p24 expression from the pNL4-3-LucE- molecular clone in 293T cells. Drugs were added where indicated to the same concentrations as in A. p24 levels were determined in cell extract at 12 hr post-transfection.