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Figure 8 | Retrovirology

Figure 8

From: Ancient, independent evolution and distinct molecular features of the novel human T-lymphotropic virus type 4

Figure 8

EMSA using a 32P-labeled probe representing the c-Myb binding site within the HTLV-4 LTR (lane 1) incubated with Jurkat nuclear extract (lanes 2–6). A 100-fold excess of unlabeled probe sequence (specific competitor, lane 3) or an unlabeled oligonucleotide containing mutations within the c-Myb binding site (non-specific competitor, lane 4) were added as indicated. Non-specific (lane 5) and Myb-specific (lane 6) antibodies were added and the supershifted band is indicated on the right panel, which is a longer exposure of the left panel.

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