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Table 5 Primers used to generate XPR1 mutants.

From: Six host range variants of the xenotropic/polytropic gammaretroviruses define determinants for entry in the XPR1 cell surface receptor

Mutant

Primer sequence (5'→3')

GenBank number

KPYK, ESTV

1F: AAATCCAGATTTTGGCTGCTCAA (1315-1337)

1R: GCAGGCACTGGATGAAGCGAA (1583-1603)

2F: TTCGCTTCATCCAGTGCCTGC (1583-1603)

2R: AAGAGACCCCAGTCCATCTTGA (1799-1820)

NM_011273

S505P

F: CACGAAGAACAAAATCACCCTGACACCGTGGTGTTCT (1705-1741)

R: AGAACACCACGGTGTCAGGGTGATTTTGTTCTTCGTG (1705-1741)

NM_011273

T507Y

F: AGAACAAAATCACTCTGACTACGTGGTGTTCTTTTA

CCTGTGG (1710-1752)

R: CCACAGGTAAAAGAACACCACGTAGTCAGAGTGATT

TTGTTCT (1710-1752)

NM_011273

P505S

F: CACAAAGAACAAAATCACTCTGACTACAAGGTGTTC

(1495-1530)

R: GAACACCTTGTAGTCAGAGTGATTTTGTTCTTTGTG

(1495-1530)

EF606903

Y507T

F: AGAACAAAATCACCCTGACACCAAGGTGTTCTTTTA

CCTGTGG (1500-1542)

R: CCACAGGTAAAAGAACACCTTGGTGTCAGGGTGATT

TTGTTCT (1500-1542)

EF606903

TV

F: AGAACAAAATCACCCTGACACCGTGGTGTTCTTTTAC

CTGTGG (1500-1542)

R: CCACAGGTAAAAGAACACCACGGTGTCAGGGTGATT

TTGTTCT (1500-1542)

EF606903

  1. Underlined letters represent introduced base substitutions. Numbers in parentheses indicate the position of each primer in the indicated GenBank sequence.