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Figure 6 | Retrovirology

Figure 6

From: The formation of cysteine-linked dimers of BST-2/tetherin is important for inhibition of HIV-1 virus release but not for sensitivity to Vpu

Figure 6

Dimerization of BST-2 is not a prerequisite for sensitivity to Vpu. 293T cells were transfected with 5 μg of wt pNL4-3 (lanes 1-4) or pNL4-3/Udel (lanes 5-8) in the absence of BST-2 (lanes 1 & 5) or together with 0.01 (lanes 2 & 6), 0.03 (lanes 3 & 7), and 0.1 μg (lanes 4 & 8) of vectors encoding cysteine mutants of BST-2 containing only one remaining cysteine (C12, C23, C13) or no cysteine at all (C3A). Twenty-four hours later, cell lysates were prepared from a fraction of the cells as described in the Methods and subjected to immunoblot analysis using a BST-2-specific antibody.

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