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Figure 4 | Retrovirology

Figure 4

From: The formation of cysteine-linked dimers of BST-2/tetherin is important for inhibition of HIV-1 virus release but not for sensitivity to Vpu

Figure 4

Glycosylation of BST-2 is not required for inhibition of virus release and for sensitivity to Vpu. (A) Analysis of wt BST-2. (B) Analysis of BST-2 N1/N2. (A & B) Cells were metabolically labeled for 90 min with [35S]-methionine as described in Methods and cell lysates and cell-free supernatants were subjected to immunoprecipitation by an HIV-positive patient serum. Immunoprecipitates were subjected to SDS-PAGE and proteins were visualized by fluorography (left panels). Virus release was quantified by phosphoimage analysis using a Fujifilm FLA7000. Virus release was calculated independently for each sample by determining the percentage of cell-free CA protein relative to the total intra- and extra-cellular Gag protein. Solid circles represent wt NL4-3. Open circles represent NL4-3/Udel. Data are presented as a function of BST-2 concentration on a semi-log plot.

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Retrovirology

ISSN: 1742-4690

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