Figure 2

Endogenous or induced expression of the LMO2 gene. (A) mRNA expression of the LMO2 gene in TPA-Mat, Jurkat, HeLa, K562 and TPA-Mat-ecoR cells. Total RNA isolated from the indicated cells was subjected to RT-PCR using the primers for LMO2 or GAPDH as a control. Aliquots of the LMO2 PCR products were subsequently subjected to the nested PCR for LMO2. The PCR products were visualized with ethidium bromide staining. Upper, middle and lower panels indicate the PCR products derived from LMO2, LMO2 and GAPDH mRNA, respectively. TPA-Mat-ecoR cells were infected with (+) or without (-) the MLV vector. (B) Induction of reporter gene activity by the insertion of MLV LTR into the HIR. Luciferase expression constructs with the MLV LTR inserted into the HIR of the LMO2 promoter region were assayed in TPA-Mat-ecoR cells. -2965 and -1798 indicate an integration site reported in the leukemia patient and a site where we found forward or reverse orientation integrated vector, respectively. Black and white arrows respectively denote the reverse and forward orientation, relative to transcription, of the integrated MLV LTRs.