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Figure 7 | Retrovirology

Figure 7

From: Mechanism of HIV-1 Tat RNA translation and its activation by the Tat protein

Figure 7

Influence of Tat-HeLa cell extracts on Tat RNA translation in the RRL. Tat-HeLa cell extracts were prepared as described in methods and increasing amounts added to the URRL. Results show that addition of increasing amounts of Tat-HeLa cell extracts enhanced translation of the Tat and genomic UTR-Rluc RNA by up to two-fold (Panel A). On the contrary, there was no effect on the translation of Rluc RNA, or of 5' UTR-Rluc RNA lacking the TAR-pA sequences (panel B). In agreement with this, translation of the TAR-pA-Rluc RNA was enhanced by up to three fold by the HeLa-Tat cell extracts (panel C). All experiments were carried out at least three times.

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