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Figure 5 | Retrovirology

Figure 5

From: Involvement of TORC2, a CREB co-activator, in the in vivo-specific transcriptional control of HTLV-1

Figure 5

Effect of the knock-down of TORC genes on the expression of Gax in EL4-Gax cells. A. Expression of TORC proteins and Gax in EL4-Gax cells transduced with retrovirus (for TORC1 and 3) or lentivirus (for TORC2) vectors encoding siRNA against each TORC gene. Expression of the siRNA resulted in the reduction of respective TORC proteins, but only siRNA to the TORC2 gene suppressed Gax gene expression. Proteins were detected with antibodies to the respective TORC proteins, Tax, or β-actin by the ECL or ECL plus system. B. Densitometric analysis data of TORC proteins and Gax protein normalized to β-actin are presented as mean ± SEM of three independent experiments. T1, TORC1; T2, TORC2; T3, TORC3.

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