Figure 4
From: Involvement of TORC2, a CREB co-activator, in the in vivo-specific transcriptional control of HTLV-1
Expression of TORC proteins in EL4-Gax cells. Anti-TORC1 (A), anti-TORC2 (B), and anti-TORC3 (C) antibodies were used to detect each protein in EL4-Gax cells grown in vitro, in vivo, and ex vivo. Equivalent protein loading was confirmed by stripping and re-probing the blot with an anti-β-actin antibody. Apparent molecular weights of marker protein are indicated. D. Phosphorylation of TORC2. Protein from EL4-Gax cells was incubated with or without rAPid Alkaline Phosphatase (see methods in detail). E. ChIP analysis of TORCs in EL4-Gax cells in vitro (a) and in vivo (b). Little or no binding of TORC1 and TORC3 to the Gax enhancer region was observed in vitro (a) or in vivo (b), but the binding of TORC2 to the Gax enhancer region was high in vitro (a) and reduced when EL4-Gax cells were grown in vivo (b).