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Figure 2 | Retrovirology

Figure 2

From: Involvement of TORC2, a CREB co-activator, in the in vivo-specific transcriptional control of HTLV-1

Figure 2

CpG methylation of the enhancer/promoter region of provirus DNA in EL4-Gax cells. Top: locations of CpG sites (#1–11) in the HTLV-1 U3 region studied in this experiment. The sense primer is complementary to the mouse genomic sequence flanking the 5'-LTR of provirus at the integration site, and the anti-sense primer is complementary to the junction sequence between the HTLV-1 and MLV U3 regions. The three 21 -bp enhancer sequences are indicated as boxes. Bottom: results of bisulfite genomic sequencing analysis of four independent experiments. Methylated and unmethylated CpG sites are expressed as filled and open rectangles, respectively. Amplified PCR products were subcloned into pGEM-T vector, and the nucleotide sequences of at least 13 clones were determined. GFP mfi: the GFP mean fluorescent intensity of EL4-Gax cells used for bisulfite genomic sequencing analysis.

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