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Figure 5 | Retrovirology

Figure 5

From: Modification of a loop sequence between α-helices 6 and 7 of virus capsid (CA) protein in a human immunodeficiency virus type 1 (HIV-1) derivative that has simian immunodeficiency virus (SIVmac239) vifand CA α-helices 4 and 5 loop improves replication in cynomolgus monkey cells

Figure 5

Replication capabilities of HIV-1 derivatives in peripheral blood mononuclear cells (PBMC) from CM. (A) PBMCs were obtained from CM, after which the CD8+ cells were removed, and the cells were stimulated with PHA-L for 1 day. (B) CD8-depleted CM PBMC were first stimulated with 1 μg/ml of PHA-L for 2 days and then with human IL2 100 U/ml for 2 more days. Equal amounts of p24 of NL-DT5R (white squares) or NL-DT5R6/7S (black squares) were inoculated, and the culture supernatants were collected periodically. p24 antigen levels were measured by ELISA. Values represent means with actual fluctuations of duplicate samples added. The values for mock infected cell culture supernatants were zero in the ELISA assay.

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