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Figure 3 | Retrovirology

Figure 3

From: Modification of a loop sequence between α-helices 6 and 7 of virus capsid (CA) protein in a human immunodeficiency virus type 1 (HIV-1) derivative that has simian immunodeficiency virus (SIVmac239) vifand CA α-helices 4 and 5 loop improves replication in cynomolgus monkey cells

Figure 3

Western blot analysis of CA and CypA in particles of HIV-1 derivatives. The viral particles of NL-SVR (lane 1), NL-SVR6/7S (lane 2), NL-ScaVR (lane 3) and NL-ScaVR6/7S (lane 4) were purified by ultracentrifugation through a 20% sucrose cushion. CypA (upper panel) and p24 and p55 proteins (lower panel) were visualized by Western blotting (WB) using anti-CypA and anti-p24 antibody, respectively. "H" and "S" denote the amino acid sequences derived from HIV-1 and SIVmac, respectively.

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