I3C inhibits growth of HUT-102 cells in SCID mice. HUT-102 cells (1 × 107 per mouse) were inoculated subcutaneously into SCID mice. The mice (n = 6/group) were treated with either vehicle or I3C (50 mg/kg given orally every day). Treatment was initiated on the day after inoculation. (A) Body weight of mice measured weekly for four weeks. (B) Photographs of an untreated mouse and I3C-treated mouse inoculated 28 days earlier with HUT-102 cells subcutaneously in the postauricular region. (C) The mice were monitored for tumor volumes at 7, 14, 21 and 28 days after cell inoculation. (D and E) Tumors removed from I3C-treated mice and untreated mice on day 28 after cell inoculation were weighed. *P < 0.05, compared with the control. (F) TUNEL assays show apoptotic cells in tumors from mice treated with vehicle control or I3C. Note the presence of only few apoptotic cells in tumors from the control mice (top panel), compared with the abundant apoptotic cells in tumors from the I3C-treated mice (bottom panel). Magnification, × 100.