Effects of I3C on the expression of cell-cycle and apoptosis regulatory proteins. Western blot analysis of HUT-102 and TL-OmI cells treated with I3C. (A) Cells were treated with I3C (100 μM) for the indicated periods. (B) Cells were treated with I3C at the indicated concentrations for 48 h. Total cellular proteins (20 μg per lane) were separated on SDS-polyacrylamide gels and transferred to the membrane. Protein levels were detected by Western blotting with antibodies directed against each protein. (C) Total RNA was extracted from HUT-102 and TL-OmI cells following treatment with I3C (100 μM) for 12 or 24 h. The mRNA expression of Tax and HBZ was analyzed by RT-PCR analysis. β-actin served as an internal control in the RT-PCR procedure. Representative data of three experiments with similar results.