Figure 3

I3C induces apoptosis of HTLV-1-infected T-cell lines. (A) Cell lines were treated with or without I3C (100 μM) for 72 h. (B) I3C induces apoptosis of HUT-102 cells in a time-dependent manner. HUT-102 cells were treated with or without I3C (100 μM) for the indicated periods. (C) I3C induces apoptosis of HUT-102 cells in a dose-dependent manner. HUT-102 cells were treated with I3C at the indicated concentrations for 72 h. Cells were harvested, then stained with the APO2.7 monoclonal antibody and analyzed by flow cytometry. Data represent the mean percentage ± SD of apoptotic cells. (D) Hoechst 33342 staining. HUT-102 cells were treated with I3C (100 μM) for 48 h and stained by Hoechst 33342. Original magnification, × 1,000.