Table 1 List of the genes and primer pairs used for qPCR and qRT-PCR experiments
Primer names forward/revers | Amplicon in figure 1A | Forward | Reverse |
|---|---|---|---|
Beta-actinF/Beta-actinR | - | AGGTCATCACCATTGGCAATG | CCCAAGAAAGATGGCTGGAA |
GAPDHF/GAPDHR | - | GGGAAGCTTACTGGAATGGCT | GGCAGGTCAGGTCAACAACA |
POMCF/POMCR | - | GCTACGGCGGCTTCATGA | CGATGGCGTTTTTGAACAGAG |
PMCHF/PMCHR | - | CGAAATGGAGACGGAACTGAA | CATCCAAGAAGCTTTCCTCAATCT |
Val_envF/Val_envR | b* | ACCCGGACATCACCCAAAG | AGTCAGAAATGCCTGCAAAAAGA |
chENV232fwd/chENV1046rev | e* | ACGGATTTCTGCCTCTCTACACA | TTCCTTGCCATGCGCGATCCC |
qPCR_envF/qPCR_envR | d* | GAAACTACCTTGTGTGCTGTCG | CGGATGTTGTGGAAAAACGA |
env277F/env353R | c* | CCCAAAATCTGTAGCCATATGC | TACGGTGGTGACAGCGGATAGG |
pol197F/pol269R | a* | TGCTTGTCTCCCCAGGGTAT | GGTGACTAAGAAAGATGAGGCGA |