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Figure 1 | Retrovirology

Figure 1

From: Proviral integrations and expression of endogenous Avian leucosis virus during long term selection for high and low body weight in two chicken lines

Figure 1

Schematic ALV genome with PCR amplicons and SNPs. A. Schematic time-line with parental generations and crosses. Generations in boxes were used for analyses in this study. Parental line generation (G) G41* and G45* were used to examine number of ALVE integrations. Expression studies were performed in the brain and peripheral tissues of G45* birds. F1* birds of the reciprocal crosses were utilized to test inheritance of ALVE genes. Eighty-two F9* birds that form the advanced intercross were utilized for the correlation studies. QTL analyses have been performed with F2** and F8** birds in the advanced intercross line [16, 53]. B. Black bar represents a complete ALVE proviral genome. Grey bars indicate PCR primers and amplicons. C. Six SNPs between HWS and LWS lines were found in the 862 bp PCR fragment e* from both genomic DNA and cDNA. a*: pol197F/pol269R. b*: Val_envF/Val_envR. c*: env277F/env353R. d*: qPCR_envF/qPCR_envR. e*: an amplicon from a primer pair chENV232fwd/chENV1046rev. See table 1.

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