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Figure 4 | Retrovirology

Figure 4

From: Cross-packaging of genetically distinct mouse and primate retroviral RNAs

Figure 4

MMTV and MPMV proteins cannot package non-specific RNAs. (A) Schematic representation of control vectors for non-specific RNA packaging of an over expressed RNA. (B) RT-PCR of cytoplasmic (upper panel) and viral (lower panel) cDNA amplified using control vector specific primers. Amplification was conducted for 30 cycles to ensure the amplification of any control vector RNA that may have been non-specifically packaged. While amplifying cytoplasmic and viral cDNAs, primers OTR637 and OTR841 were used for pTR174 and should amplify 368 bp fragment. For pAG001, primers OTR641 and OTR517 were used and should amplify 301 bp fragment.

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