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Figure 4 | Retrovirology

Figure 4

From: Pathogenic infection of Macaca nemestrinawith a CCR5-tropic subtype-C simian-human immunodeficiency virus

Figure 4

Selective loss of CD4+ T-cell subsets in the peripheral blood of R5 SHIV-C-infected pig-tailed macaques. Flow cytometric evaluation of (A) total CD4+ T cells and subsets based on expression of (B) CCR5+ and (C) CD28-CD95+ (TEM) was done using whole blood (for total CD4+ T cells) or total lymphocytes isolated from peripheral blood (for subsets). Analysis was performed by gating first on lymphocytes and then CD3+CD4+ T cells. Total absolute counts of CD4+ T cells were calculated by multiplying the percentage of CD3+CD4+ T cells by the number of total lymphocytes/ml from complete blood count (CBC) analysis. Further multiplication of the percentage of cells expressing CCR5+ or CD28-CD95+ was done to calculate the absolute counts of the subsets. The dashed line in (A) indicates 200 cells/μl, the threshold level defining human AIDS.

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