Figure 1

Ex vivo infection of lymphoid tissue by SIV isolates obtained from singly-infected or HHV-6A-coinfected macaques. Blocks of human (A, B) or macaque (C) lymphoid tissue were inoculated with different SIV isolates, and viral replication was evaluated by measuring the level of p27 antigen accumulated in the culture medium every 3 days over 12 days of culture. For each donor, 27 tissue blocks were inoculated. The data indicate the mean values (± SEM) of SIV replication. A. Replication in human lymphoid tissue of SIV isolated from singly-infected macaques (#301 n = 11; #303 n = 15; #307, n = 12) and HHV-6A-coinfected macaques (#313, n = 15; #315, n = 11; #316, n = 15; #317, n = 13). B. Comparison of the viral replication levels in tissues infected SIV isolates derived from singly-infected animals (n = 37) versus those derived from HHV-6A-coinfected animals (n = 51). The data represent the mean level of replication (± SEM) for all the isolates tested in each group. * = P < 0.001. C. Replication of SIV #303, derived from a singly-infected animal (black line), and SIV #316, derived from an HHV-6A-coinfected animal (grey line), in macaque lymphoid tissue (n = 3).