Figure 8
Effect of fadrozole treatment on Ovex1 expression in the female left gonad at E14. Chicken embryos were injected with fadrozole in ovo at E4. Fibronectin (FN) was revealed by immunofluorescence, and expression of Ovex1, Sox9 and AMH was detected by in situ hybridization of digoxigenin-labeled RNA probes on serial transverse cryostat sections of the masculinized left female gonad. Fibronectin and Sox9 are double labeling of the same section. Scale bar = 100 μm.