Figure 7

Expression profiles of Ovex1 and gonad markers in the left ovary before and after folliculogenesis. Fibronectin (FN) is revealed by immunofluorescence and gene expression detected by in situ hybridization of digoxigenin-labeled RNA probes on transverse cryostat sections. (A) Left ovary at E14. Immunostaining of fibronectin (FN) and expression of Ovex1, Lhx9, ER-α, Cvh, Stra8, FoxL2 and AMH in gonad serial sections. Fibronectin and Cvh are double labeling of the same section. (B) Left ovary at hatching (P1). Immunostaining of fibronectin (FN) and expression of Ovex1 (two aspects of the cortex desquamation process are presented), FoxL2 and Wnt4. Fibronectin and FoxL2 are double labeling of the same section. Arrows indicate FoxL2-positive cells located on the cortical side of the fibronectin cortico-medullar interface. (C) Left ovary 7 days after hatching (P7). Immunostaining of fibronectin (FN) and expression of Ovex1 (two aspects of the folliculogenesis are presented), FoxL2 and Wnt4. Fibronectin and FoxL2 are double labeling of the same section. (D) Left ovary at P14. Expression of Ovex1, FoxL2 and AMH in serial sections. (E) Left ovary at P28. Expression of Ovex1, FoxL2 and AMH in serial sections. Scale bars = 100 μm.