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Figure 8 | Retrovirology

Figure 8

From: Intracellular interactions between APOBEC3G, RNA, and HIV-1 Gag: APOBEC3G multimerization is dependent on its association with RNA

Figure 8

Characterization of HIV-1 Gag BiFC constructs and interactions with A3G. (A) Structure of HIV-1 Gag BiFC constructs. NC*, RNA-binding defective mutant of HIV-1 Gag. (B) Western blot analysis of HIV-1 Gag expression from BiFC constructs (anti-HIV-Gag polyclonal antibody) and α-tubulin in transfected 293T cells. (C) BiFC assays to evaluate interactions between HIV-1 Gag and wild-type A3G. (D) BiFC assays to evaluate interactions between HIV-1 Gag and CD1 or CD2 domain mutants of A3G.

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Retrovirology

ISSN: 1742-4690

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