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Figure 2 | Retrovirology

Figure 2

From: Intracellular interactions between APOBEC3G, RNA, and HIV-1 Gag: APOBEC3G multimerization is dependent on its association with RNA

Figure 2

Reconstitution of YFP fluorescence with A3G BiFC constructs. (A) Reconstitution of fluorescence upon cotransfection with A3G BiFC constructs. HeLa cells were cotransfected with A3G BiFC constructs and mRFP expression plasmid to identify transfected cells. Fluorescence was reconstituted upon co-transfection with A3G-NY + A3G-CY (I), NY-A3G + CY-A3G (II), A3G-NY + CY-A3G (III), and NY-A3G + A3G-CY (IV). Transfection with A3G-NY (V), A3G-CY (VI), NY-A3G (VII), or CY-A3G (VIII) did not produce YFP fluorescence. (B) Quantfication of BiFC using flow cytometry analysis. 293T cells were co-transfected with A3G BiFC constructs and mRFP expression plasmid as an internal control for transfection, and the percentage of YFP+ cells in mRFP+ cells was determined. The percentage of YFP+ cells in mRFP+ cells after co-transfection with A3G-NY and A3G-CY (15.3%) was set to 100%. The error bars represent the s.e.m. of two independent experiments.

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Retrovirology

ISSN: 1742-4690

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