Figure 6

Reporter length, target distance, matched and overlapping hairpin pairs. (a) The suppressive activities of hairpins that were assayed with both long core gene reporters and shorter core gene fragment reporters, were plotted and grouped according to reporter length. (b) Suppressive activity was plotted against the distance of the target site from the fusion junction for all hairpins tested. (c) The suppressive activities of 14 matched pairs of hairpins with 20 and 21 bp stems designed to the same target sites. (d) The suppressive activities of 73 pairs of neighboring hairpins with target sites a single nucleotide apart.